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Our Sequencing Platforms
The addition of the HiSeq 2500 to our portfolio of sequencers allows us to better serve you, our customers. The innate flexibility of this instrument provides us with the…
Illumina’s MiSeq is quickly becoming the sequencer of choice for researchers, such as yourself, to carryout their genomic and targeted resequencing studies. The highly experienced team at MR DNA is…
The Titanium series reagents for the 454 sequencer help provide longer base-pair reads and higher sequencing read density than previous improvements. This has greatly assisted in the use of high…
Ion Torrent PGM
The Ion PGM supplies an innovative approach to sequencing technology. Utilizing the semiconductor technology employed by the PGM, the scientists at MR DNA are able to provide you, our customer, with…
The 16s rRNA gene is the ideal target to complete metagenomic studies. Our in-house 16s sequencing primers are designed to target the nine hypervariable regions. MRDNA amplicon sequencing methods are not limited to bacteria, but include fungi, archaea, and eukaryotes.
We offer a wide range of NGS platforms, making whole genome sequencing all the more affordable. From small microbial genomes to larger eukaryote genomes, whole genome sequencing provides researchers the opportunity to identify disease causing variants without the labor of traditional microbiology techniques.
MRDNA is passionate about microbiome research. In addition to our 16s sequencing service, we also offer Shotgun metagenome sequencing services. From targeted taxonomic analysis to a complete overview metabolic pathways, allow us to combine our experience in mirobiome analysis to your research project.
Gut microbiota isolated from the European pond turtle (Emys orbicularis) and its antimicrobial resistance.
The aim of the study was to isolate cultivable gut microbiota from European pond turtles kept at the Lithuanian Zoo and to determine antimicrobial resistance of the isolates. The study subjects included 8 elderly turtles living at the Lithuanian Zoo for about 50 years as well as their offspring - 24 young individuals (1-2 years old) that were hatched at the same zoo. Animals were not exposed by treatment with antimicrobials during the last 3 years. Gut samples were taken from the cloaca and inoculated onto universal media. Isolates then were identified using sequence analysis of 16S rRNA. The antimicrobial susceptibility testing was performed using the agar diffusion method according to Kirby-Bauer. Clinical breakpoints according to CLSI whenever possible, were used for interpretation of susceptibility. Bacterial isolates resistant to at least three antimicrobials of different classes were treated as multi-resistant. Fifty-two bacterial isolates were obtained and identified from turtle gut samples. The most prevalent genera included Aeromonas, Chryseobacterium and Citrobacter. Fifty percent of the isolates obtained from elderly turtles (CI 95% - 19.01-80.99) and 54.8% (CI 95% - 39.75-69.85) of the isolates from young animals were identified as multi-resistant. The most common resistance rates of the isolates from both groups of the turtles were observed toward ampicillin (86.6%), ciprofloxacin (61.5%) and gentamicin (40.4%). The lowest number of resistant isolates were detected toward combination of sulfamethoxazole-trimethoprim (26.9%). The study revealed that European pond turtles kept in captivity are carriers of multi-resistant bacteria however, further studies need to be performed to investigate whether the resistant microorganisms are natural microbiota for this species or they were acquired in the zoo.
Ruzauskas M, Misyte S, Vaskeviciute L, et al. Gut microbiota isolated from the European pond turtle (Emys orbicularis) and its antimicrobial resistance. Pol J Vet Sci. 2016;19(4):723-730.
In The News
January 17, 2017
One of the key areas of metagenomic and microbiome research has been on the transmission of bacteria from mother to infant . Asnicar et al., recently published a study where they sampled 5 mother-infant pairs collecting both fecal and breast milk samples. In order to characterize the microbiome of each pair, DNA was extracted and prepared for metagenomic sequencing on the Illumina HiSeq 2500. Both breast feeding and delivery method have both been seen to play an important role in the diversity of the microbiome of infants relative to their mothers. A vaginal delivery has been documented as providing the infant with a much more diverse microbiome thereby decreasing the likelihood of the infant from succumbing to certain health risks a newborn would experience following a C-section. The research team from Italy postulates that the seemingly apparent transmission of bacteria from mother to infant based on species commonality may not be the most thorough investigative method. "It is therefore imperative to assess whether shared microbes belong to the same genetic variant (i.e., strain) to support the hypothesis of vertical transmission.", states Asnicar et al. Through shotgun metagenomics, the research team was able to assess that based on strain similarity, there does appear to be evidence in support of vertical transmission of the mother's microbiome to their infant. Even as early as 3 months does the microbiome of the infant begin to shift to to what the research team describes as "mother-like".
Source: Studying Vertical Microbiome Transmission from Mothers to Infants by Strain-Level Metagenomic Profiling. mSystems. 2017;2(1):e00164-16.
January 16, 2017
Have we entered a new era DNA sequencing? An era where DNA sequencing has become so commonplace that the curiosity of the general public is able to inspire new scientific studies. An era of "cultural genomics". The latest study of the Canadian beaver seems to be one of the first endeavors in "cultural genomics." The research team, based in Toronto, states , "We release the beaver genome to mark Canada’s sesquicentennial, and hope the initiative will catalyze other exploratory investigations in “cultural genomics,” of which this project was motivated by a nation’s curiosity and the pride in the animal that has most shaped its history." The world of DNA sequencing and genomics has come a long way since the human genome project. Popularized by "CSI" TV shows and citizen science projects, the general public has become very familiar with the overall concept of DNA sequencing and the capabilities available to the scientific community. As DNA sequencing methods, such as whole genome sequencing, continue to drop in price, the more the scientific community can take an interest in the concerns and ideas of their surrounding communities and investigate topical issues like the history of the Canadian beaver. Lok et al., utilized both the PacBio RSII and Illumina HiSeq X systems to preform de novo genome and de novo transcriptome sequencing. Both of these technologies have allowed the cost of genome and transcriptome sequencing to decrease dramatically and the sequencing power to increase. The results of the genome assembly generated by the Illumina HiSeq X indicate that the research team was able to achieve 160x coverage of the 4 billion bp beaver genome. As sequencing power and popularity continue to progress, it is not difficult to imagine whole genome sequencing becoming a routine test ordered by your family physician.
Source: Lok S, Paton TA, Wang Z, et al. De novo genome and transcriptome assembly of the Canadian beaver (Castor canadensis). G3 (Bethesda). 2017;
In The News
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